Development of ciprofloxacin resistance due to chromosomal mutations induced by 2-nitrofluorene.
نویسندگان
چکیده
In this paper we investigated the ability of 2-nitrofluorene to induce mutations leading to antibiotic resistance in quinolone-sensitive strain Salmonella typhimurium. After preincubation of bacteria with 2-nitrofluorene, the frequency of mutation to ciprofloxacin resistance was 57 fold higher than in the case of spontaneous mutability. Some of resultant resistant colonies showed a great increase of ciprofloxacin MIC.
منابع مشابه
The effect of environmental pollutants and food processing on the development of antibiotic resistance.
AIM The purpose of this study was to determine how various compounds known to be positive mutagens, contribute to the development of mutations leading to ciprofloxacin resistance in Salmonella enterica subsp. enterica serotype Typhimurium. The molecular mechanism of ciprofloxacin resistance in treated strains was investigated. METHODS A modified version of the incorporation plate test was use...
متن کاملEvaluation of Resistance to Fluoroquinolones and Its Relationship whit parC Gene Mutation in Klebsiella pneumoniae Clinical Isolates
Background & Objective: Klebsiella pneumoniae has received attention due to a wide range of diseases and antibiotic resistance.The resistance to fluoroquinolones in gram-negative bacteria is often due to chromosomal mutations in the gyr and par genes. This research aimed at investigating the pattern of fluoroquinolone resistance and its relation with a mutation in the parC gene among clinical i...
متن کاملRole of PA3574 (nalD) gene in development of ciprofloxacin resistance in Pseudomonas aeruginosa isolates
Background & Aims: Pseudomonas aeruginosa is a gram-negative opportunistic pathogen and one of the mortality causes of nosocomial infections. One of the drug resistance mechanisms in P.aeruginosa is mutation in negative regulator genes of mexAB-oprM efflux pump system such as nalD. The aim of this study was to investigate the role of nalD mutations in P. aeruginosa isolates of Guilan province i...
متن کاملThe involvement of mutation in the serine 83 of quinolone resistant determining regions of the GyrA Gene in resistance to ciprofloxacin in Escherichia coli .
Appearance of bacteria resistant to antibacterial agents puts physicians in trouble and threatens the health of the world. The rapid development of bacterial resistance in Escherichia coli to ciprofloxacin makes difficult the treatment of infectious diseases. So, detection of the locations of possible mutations in gyrase A gene ( gyrA ) in these mutants is very important to determine the mech...
متن کاملAssociation between mutations in gyrA and parC genes of Acinetobacter baumannii clinical isolates and ciprofloxacin resistance
Objective(s): We investigated the contribution of gyrA and parC mutational mechanism in decreased ciprofloxacin susceptibility of Acinetobacter baumannii isolated from burn wound infections. Materials and Methods: Ciprofloxacin susceptibility of 50 A. baumannii isolates was evaluated by disk diffusion and agar dilution methods. PCR and sequencing were performed for detection of mutation in gyr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia
دوره 149 2 شماره
صفحات -
تاریخ انتشار 2005